Gene Editing services

 

The company’s scientific capabilities cover all facets of Gene Editing, offering a wide range of support for molecular biology work, including but not limited to:

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• Gene synthesis

• Subcloning & PCR cloning

• Plasmid preparation (up to milligram scale)

• Mutagenesis

• CRISPR sgRNA design and synthesis

• CRISPR repair template design and synthesis

• CRISPR edit validation through Amplicon sequencing

• Protein expression and purification in multiple systems (E.coli, yeast, and mammalian cells)

• Protein design and engineering

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The company is capable of full fledged lipid Nanoparticle synthesis to enable targeted delivery of mRNA, Oligonucleotides and CRISPR constructs to specific cell / tissues.

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Lipid Nanoparticle Synthesis and Encapsulation Service

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  Wisecorner Laboratories Pvt Ltd offers full range of services from Nucleic acid design and synthesis till its encapsulation using Lipid Nanoparticles followed by its analytical characterization.

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 Lipid nanoparticles (LNPs) are the most clinically advanced non-viral gene delivery system. Lipid nanoparticles safely and effectively deliver nucleic acids, overcoming a major barrier preventing the development and use of genetic medicines. Genetic medicine has many different applications such as gene editing, rapid vaccine development, immuno-oncology and treatment of rare genetic and undruggable diseases; all of which are usually hindered by nucleic acid delivery inefficiency.

                        

Lipid nanoparticles offer many advantages over previous lipid-based nucleic acid delivery systems including:

 

• High nucleic acid encapsulation efficiency and potent transfection
• Improved penetration into tissues to deliver therapeutics
• Low cytotoxicity and immunogenicity

 

These characteristics make lipid nanoparticles excellent candidates for nucleic acid delivery.

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