top of page

Gene Editing

 

The company’s scientific capabilities cover all facets of Gene Editing, offering a wide range of support for molecular biology work, including but not limited to:

​

  • Gene synthesis

  • Subcloning & PCR cloning

  • Plasmid preparation (up to milligram scale)

  • Mutagenesis

  • CRISPR sgRNA design and synthesis

  • CRISPR repair template design and synthesis

  • CRISPR edit validation through Amplicon sequencing

  • Protein expression and purification in multiple systems (E.coli, yeast, and mammalian cells)

  • Protein design and engineering

​

The company is capable of full fledged lipid Nanoparticle synthesis to enable targeted delivery of mRNA, Oligonucleotides and CRISPR constructs to specific cell / tissues.

Lipid Nanoparticle Synthesis and Encapsulation Service

  Wisecorner Laboratories Pvt Ltd offers full range of services from mRNA synthesis till its encapsulation using Lipid Nanoparticles followed by its analytical characterization.

​

 Lipid nanoparticles (LNPs) are the most clinically advanced non-viral gene delivery system. Lipid nanoparticles safely and effectively deliver nucleic acids, overcoming a major barrier preventing the development and use of genetic medicines. Genetic medicine has many different applications such as gene editing, rapid vaccine development, immuno-oncology and treatment of rare genetic and undruggable diseases; all of which are usually hindered by nucleic acid delivery inefficiency.

                        

Lipid nanoparticles offer many advantages over previous lipid-based nucleic acid delivery systems including:

 

• High nucleic acid encapsulation efficiency and potent transfection
• Improved penetration into tissues to deliver therapeutics
• Low cytotoxicity and immunogenicity

 

These characteristics make lipid nanoparticles excellent candidates for nucleic acid delivery.

​

bottom of page